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Laboratorio de Biología Estructural y Molecular (BEM)

Centro de Equipamiento Mayor (CEM)

Facultad de Ciencias

Universidad de Chile

Las Palmeras 3425

Ñuñoa, Santiago

Chile

cem@uchile.cl

+56 2 29787380

Visitors:

Publicaciones Lagos R. (últimos diez años)

Binding of dihydroxynaphthyl aryl ketones to tubulin colchicine site inhibits microtubule assembly.

October 23, 2015

Gutierrez E, Benites J, Valderrama JA, Calderon PB, Verrax J, Nova E, Villanelo F, Maturana D, Escobar C, Lagos R, Monasterio O.
Biochem Biophys Res Commun. 2015 Oct 23;466(3):418-25. doi: 10.1016/j.bbrc.2015.09.041.

Single tryptophan mutants of FtsZ: nucleotide binding/exchange and conformational transitions.

July, 2014

Montecinos-Franjola F, James NG, Concha-Marambio L, Brunet JE, Lagos R, Monasterio O, Jameson DM.
Biochim Biophys Acta. 2014 Jul;1844(7):1193-200. doi:10.1016/j.bbapap.2014.03.012.

Glutamate 83 and arginine 85 of helix H3 bend are key residues for FtsZ polymerization, GTPase activity and cellular viability of Escherichia coli: lateral mutations affect FtsZ polymerization and E. coli viability.

February 5, 2013

Shin JY, Vollmer W, Lagos R, Monasterio O.
BMC Microbiol. 2013 Feb 5;13:26. doi: 10.1186/1471-2180-13-26.

Studies on the dissociation and urea-induced unfolding of FtsZ support the dimer nucleus polymerization mechanism.

May 2, 2012

Montecinos-Franjola F, Ross JA, Sánchez SA, Brunet JE, Lagos R, Jameson DM, Monasterio O.
Biophys J. 2012 May 2;102(9):2176-85. doi: 10.1016/j.bpj.2012.03.064.

Structural characterization of microcin E492 amyloid formation: identification of the precursors.

April, 2012

Arranz R, Mercado G, Martín-Benito J, Giraldo R, Monasterio O, Lagos R, Valpuesta JM.
J Struct Biol. 2012 Apr;178(1):54-60. doi: 10.1016/j.jsb.2012.02.015.

A model for the Escherichia coli FtsB/FtsL/FtsQ cell division complex.

June, 2011

Villanelo F, Ordenes A, Brunet J, Lagos R, Monasterio O.
BMC Struct Biol. 2011 Jun 14;11:28. doi: 10.1186/1472-6807-11-28.

The production in vivo of microcin E492 with antibacterial activity depends on salmochelin and EntF.

August, 2008

Mercado G, Tello M, Marín M, Monasterio O, Lagos R.
J Bacteriol. 2008 Aug;190(15):5464-71. doi: 10.1128/JB.00351-08.

4',6-diamidino-2-phenylindole (DAPI) induces bundling of Escherichia coli FtsZ polymers inhibiting the GTPase activity.

September, 2007

Nova E, Montecinos F, Brunet JE, Lagos R, Monasterio O.
Arch Biochem Biophys. 2007 Sep 15;465(2):315-9.

Domain folding and flexibility of Escherichia coli FtsZ determined by tryptophan site-directed mutagenesis.

August, 2007

Díaz-Espinoza R, Garcés AP, Arbildua JJ, Montecinos F, Brunet JE, Lagos R, Monasterio O.
Protein Sci. 2007 Aug;16(8):1543-56.

Microcin E492 amyloid formation is retarded by posttranslational modification.

July 8, 2013

Marcoleta A, Marín M, Mercado G, Valpuesta JM, Monasterio O, Lagos R.
J Bacteriol. 2013 Sep;195(17):3995-4004. doi: 10.1128/JB.00564-13

Whole-genome sequence of the microcin E492-producing strain Klebsiella pneumoniae RYC492.

May 9, 2013

Marcoleta A, Gutiérrez-Cortez S, Maturana D, Monasterio O, Lagos R.
Genome Announc. 2013 May 9;1(3). pii: e00178-13. doi: 10.1128/genomeA.00178-13

Klebsiella pneumoniae asparagine tDNAs are integration hotspots for different genomic islands encoding microcin E492 production determinants and other putative virulence factors present in hypervirulent strains.

June 3, 2016

Marcoleta AE, Berríos-Pastén C, Nuñez G, Monasterio O, Lagos R.
Front Microbiol. 2016 Jun 3;7:849. doi:10.3389/fmicb.2016.00849

Identification of key amino acid residues modulating intracellular and in vitro microcin E492 amyloid formation.

January 28, 2016

Aguilera P, Marcoleta A, Lobos-Ruiz P, Arranz R, Valpuesta JM, Monasterio O, Lagos R.
Front Microbiol. 2016 Jan 28;7:35. doi: 10.3389/fmicb.2016.00035