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Laboratorio de Biología Estructural y Molecular (BEM)

Centro de Equipamiento Mayor (CEM)

Facultad de Ciencias

Universidad de Chile

Las Palmeras 3425

Ñuñoa, Santiago

Chile

cem@uchile.cl

+56 2 29787380

Visitors:

Lagos R. publications (last 10 years).

The Ferric uptake regulator (Fur) and iron availability control the production and maturation of the antibacterial peptide microcin E492.

Accepted for publication in PLoS one

Marcoleta, AE, Gutiérrez-Cortez, S., Hurtado, F., Argandoña, Y., Corsini, G., Monasterio, O., and Lagos, R.
PLoS one (accepted).

Evaluating different virulence traits of Klebsiella pneumoniae using the social amoeba Dictyostelium discoideum and zebrafish larvae as host models.

March, 2018

Marcoleta, AE, Varas, M.A, Ortiz-Severín, J. Vásquez, L., Berríos-Pastén,  C.,  Sabag, A., Chávez,  F.P., Allende, M.L., Santiviago, C.,  Monasterio, O.,  and Lagos, R.
Frontiers in Cellular and Infection Microbiology 8:30. doi: 10.3389/fcimb.2018.00030.

Thermal adaptation of mesophilic and thermophilic FtsZ assembly by modulation of the critical concentration.

October, 2017

Concha-Marambio L, Maldonado P, Lagos R, Monasterio O, Montecinos-Franjola F.
PLoS One. 2017 Oct 5;12(10):e0185707. doi: 10.1371/journal.pone.0185707.

Mutations on FtsZ lateral helix H3 that disrupt cell viability hamper reorganization of polymers on lipid surfaces.

June 20, 2017

Marquez, I.F.,  Mateos-Gil, P.,  Shin, J-Y., Lagos, R.,  Monasterio, O.,  Velez, M.
Biochim Biophys Acta  – Biomembranes. 1859, 1815-1827.

Inhibition of cancer cell growth and migration by dihydroxynaphthyl aryl ketones.

September, 2016

Benites, J.,  Valderrama, J.A., Ríos, D.,  Lagos, R., Monasterio, O., and  Buc Calderon, P.
Molecular & Cellular Toxicology  (MCT) 12:  237-242. doi:10.1007/s13273-016-0028-8

Klebsiella pneumoniae asparagine tDNAs are integration hotspots for different genomic islands encoding microcin E492 production determinants and other putative virulence factors present in hypervirulent strains.

June 3, 2016

Marcoleta AE, Berríos-Pastén C, Nuñez G, Monasterio O, Lagos R.
Front Microbiol. 2016 Jun 3;7:849. doi:10.3389/fmicb.2016.00849

Identification of key amino acid residues modulating intracellular and in vitro microcin E492 amyloid formation.

January 28, 2016

Aguilera P, Marcoleta A, Lobos-Ruiz P, Arranz R, Valpuesta JM, Monasterio O, Lagos R.
Front Microbiol. 2016 Jan 28;7:35. doi: 10.3389/fmicb.2016.00035

Binding of dihydroxynaphthyl aryl ketones to tubulin colchicine site inhibits microtubule assembly.

October 23, 2015

Gutierrez E, Benites J, Valderrama JA, Calderon PB, Verrax J, Nova E, Villanelo F, Maturana D, Escobar C, Lagos R, Monasterio O.
Biochem Biophys Res Commun. 2015 Oct 23;466(3):418-25. doi: 10.1016/j.bbrc.2015.09.041.

Single tryptophan mutants of FtsZ: nucleotide binding/exchange and conformational transitions.

July, 2014

Montecinos-Franjola F, James NG, Concha-Marambio L, Brunet JE, Lagos R, Monasterio O, Jameson DM.
Biochim Biophys Acta. 2014 Jul;1844(7):1193-200. doi:10.1016/j.bbapap.2014.03.012.

Bacteriocins. Brenner’s Online Encyclopedia of Genetics, Second edition, Vol .1

2013

Lagos R.
Brenner’s Online Encyclopedia of Genetics, Second edition, Vol.1 (Maloy, S. and Hughes, K., Editors) Academic Press. San Diego, USA, pp. 277-279.

Microcin E492 amyloid formation is retarded by posttranslational modification.

July 8, 2013

Marcoleta A, Marín M, Mercado G, Valpuesta JM, Monasterio O, Lagos R.
J Bacteriol. 2013 Sep;195(17):3995-4004. doi: 10.1128/JB.00564-13

Whole-genome sequence of the microcin E492-producing strain Klebsiella pneumoniae RYC492.

May 9, 2013

Marcoleta A, Gutiérrez-Cortez S, Maturana D, Monasterio O, Lagos R.
Genome Announc. 2013 May 9;1(3). pii: e00178-13. doi: 10.1128/genomeA.00178-13

Glutamate 83 and arginine 85 of helix H3 bend are key residues for FtsZ polymerization, GTPase activity and cellular viability of Escherichia coli: lateral mutations affect FtsZ polymerization and E. coli viability.

February 5, 2013

Shin JY, Vollmer W, Lagos R, Monasterio O.
BMC Microbiol. 2013 Feb 5;13:26. doi: 10.1186/1471-2180-13-26.

Studies on the dissociation and urea-induced unfolding of FtsZ support the dimer nucleus polymerization mechanism.

May 2, 2012

Montecinos-Franjola F, Ross JA, Sánchez SA, Brunet JE, Lagos R, Jameson DM, Monasterio O.
Biophys J. 2012 May 2;102(9):2176-85. doi: 10.1016/j.bpj.2012.03.064.

Structural characterization of microcin E492 amyloid formation: identification of the precursors.

April, 2012

Arranz R, Mercado G, Martín-Benito J, Giraldo R, Monasterio O, Lagos R, Valpuesta JM.
J Struct Biol. 2012 Apr;178(1):54-60. doi: 10.1016/j.jsb.2012.02.015.

A model for the Escherichia coli FtsB/FtsL/FtsQ cell division complex.

June, 2011

Villanelo F, Ordenes A, Brunet J, Lagos R, Monasterio O.
BMC Struct Biol. 2011 Jun 14;11:28. doi: 10.1186/1472-6807-11-28.

 Bacterial Tubulins A and B Exhibit Polarized Growth, Mixed-Polarity Bundling, and Destabilization by GTP Hydrolysis. 

October, 2017

Díaz-Celis C, Risca VI, Hurtado F, Polka JK, Hansen SD, Maturana D, Lagos R, Mullins RD, Monasterio O.
J Bacteriol. 2017 Sep 5;199(19). pii: e00211-17. doi: 10.1128/JB.00211-17.

Antibacterial and antitumorigenic properties of microcin E492, a pore forming bacteriocin.

January, 2009

Lagos, R., Tello, M., Mercado, G., García, V. and Monasterio, O.
Curr. Pharmaceutical Biotech.  10, 74-85.

The production in vivo of microcin E492 with antibacterial activity depends on salmochelin and EntF.

August, 2008

Mercado G, Tello M, Marín M, Monasterio O, Lagos R.
J Bacteriol. 2008 Aug;190(15):5464-71. doi: 10.1128/JB.00351-08.