BEM
Laboratorio de Biología Estructural y Molecular
Universidad de Chile
Lagos R. publications (last 10 years).
Identification of aggregation-prone and gatekeeper residues in bacterial amyloids using site-directed mutagenesis and flow cytometry.
2022
Aguilera, P., Marín, J., Lagos, R., Marcoleta, A.
In Methods in Molecular Biology 2538. Bacterial Amyloids: Methods and Protocols. Eds: V. Arluison, F. Wien, A. Marcoleta. Humana New York, NY. DOI: https://doi.org/10.1007/978-1-0716-2529-3
Assessment of intracellular amyloid formation in fixed and live bacteria using fluorescence microscopy.
2022
Marín, J., Aguilera, P., Lagos,R., Marcoleta, A.
In Methods in Molecular Biology 2538. Bacterial Amyloids: Methods and Protocols. Eds: V. Arluison, F. Wien, A. Marcoleta. Humana New York, NY. DOI: https://doi.org/10.1007/978-1-0716-2529-3
The highly diverse Antarctic Peninsula soil microbiota as a source of novel resistance genes.
2022
Marcoleta, AE., Arros, P., Varas, MA, Costa, J., Rojas-Salgado, J., Berríos-Pastén, C., Tapia-Fuentes, S., Silva, D., Fierro, J., Canales, N., Chávez, FP., Gaete, A., González, M., Allende, ML, Lagos, R.
Science of the Total Environment. 810 (2022) 152003. https://doi.org/10.1016/j.scitotenv.2021.152003
Exploiting zebrafish xenografts for testing the in vivo antitumorigenic activity of microcin E492 against human colorectal cancer cells.
2020
Varas, M.A., Muñoz-Montecinos, C., Kallenz, V., Simon, V., Allende, M.A., Marcoleta, A.E. and Lagos, R.
Science of the Total Environment. 810 (2022) 152003. https://doi.org/10.1016/j.scitotenv.2021.152003
Properties of genes encoding transfer RNAs as integration sites for genomic islands and prophages in Klebsiella pneumoniae.
2020
Berríos-Pastén, C., Acevedo, R., Arros, P., Varas, MA., Wyres, KL., Lam, MMC., Holt, KE, Lagos, R., AE. Marcoleta
bioRxiv preprint doi: https://doi.org/10.1101/2020.11.02.365908
Inhibition of Escherichia coli and Bacillus subtilis FtsZ Polymerization and Bacillus subtilis Growth by Dihydroxynaphtyl Aryl Ketones.
2019
Araya G, Benites J, Reyes JS, Marcoleta AE, Valderrama JA, Lagos R and Monasterio O
Frontiers in Microbiology.10:1225. doi: 10.3389/fmicb.2019.01225
Bacterial Amyloids.
2019
Marcoleta, A., Wein, F., Arluison, V., Lagos, R., Giraldo, R.
In eLS, John Wiley & Sons. Ltd: Chichester. doi: 10.1002/9780470015902.a0028401
The Ferric uptake regulator (Fur) and iron availability control the production and maturation of the antibacterial peptide microcin E492.
2018
Marcoleta, AE., Gutiérrez-Cortez, S., Hurtado, F., Argandoña, Y., Corsini, G., Monasterio, O., and Lagos, R.
PLoS ONE 13(8): e0200835. https://doi.org/10.1371/journal.pone.0200835
Evaluating different virulence traits of Klebsiella pneumoniae using the social amoeba Dictyostelium discoideum and zebrafish larvae as host models.
2018
Marcoleta, A.E, Varas, M.A, Ortiz-Severín, J. Vásquez, L., Berríos-Pastén, C., Sabag, A., Chávez, F.P., Allende, M.L., Santiviago, C., Monasterio, O., and R. Lagos.
Frontiers in Cellular and Infection Microbiology 8:30. doi: 10.3389/fcimb.2018.00030
Thermal Adaptation of Mesophilic and Thermophilic FtsZ Assembly by Modulation of the Critical Concentration.
2017
Concha-Marambio, L., Maldonado, O., Lagos, R., Monasterio, O., Montecinos-Franjola, F.
PloS ONE.(10): PLoS e0185707. https://doi.org/10.1371/journal.pone.0185707
Bacterial Microtubules Exhibit Polarized Growth, Mixed-Polarity Bundling, and Destabilization by GTP Hydrolysis.
2017
Díaz-Celis, C., Risca, V.I., Hurtado, F., Polka, J., Hansen, S.D., Maturana, D., Lagos, R., Mullins, D., Monasterio, O.
Journal of Bacteriology 199 (19) e0221-17. https://doi.org/10.1128/JB.00211-17.
Mutations on FtsZ lateral helix H3 that disrupt cell viability hamper reorganization of polymers on lipid surfaces.
2017
Marquez, I.F., Mateos-Gil, P., Shin, J-Y., Lagos, R., Monasterio, O., Velez, M.
Biochim Biophys Acta – Biomembranes. 1859, 1815-1827. https://doi.org/10.1016/j.bbamem.2017.06.009
Inhibition of cancer cell growth and migration by dihydroxynaphthyl aryl ketones.
2016
Benites, J., Valderrama, J.A., Ríos, D., Lagos, R., Monasterio, O., and Buc Calderon, P.
Molecular & Cellular Toxicology (MCT) 12: 237-242. doi:10.1007/s13273-016-0028-8
Klebsiella pneumoniae asparagine tDNAs are integration hotspots for different genomic islands encoding microcin E492 production determinants and other putative virulence factors present in hypervirulent strains.
2016
Marcoleta, A., Berríos-Pastén, C., Nuñez, G., Monasterio, O., Lagos, R.
Frontiers in Microbiology. 7:849 doi: 10.3389/fmicb.2016.00849
Identification of key amino acid residues modulating intracellular and in vitro microcin E492 amyloid formation.
2016
Aguilera, P., Marcoleta, A., Lobos-Ruiz, P., Arranz, R., Valpuesta, JM., Monasterio, O., Lagos, R.
Frontiers in Microbiology. 7:35. doi: 10.3389/fmicb.2016.00035.
Binding of dihydroxynaphtyl aryl ketones to tubulin colchicine sites inhibits microtubule assembly.
2015
Gutiérrez , E., Benites, J., Valderrama, J., Buc-Calderon, P., Verrax, J., Nova E., Villanelo, F., Maturana, D., Escobar, C., Lagos, R., Monasterio, O.
Biochem. Biophys Res. Commun. 466: 418-425. doi: 10.1016/j.bbrc.2015.09.041.
Single tryptophan mutants of FtsZ: nucleotide binding/exchange and conformational transitions.
2014
Montecinos-Franjola, F., James, N.G., Concha-Marambio, L., Brunet, J.E., Lagos, R., Monasterio, O., Jameson, D.M.
. Biochim Biophys Acta 1844, 1193-200.
Microcin E492 amyloid formation is retarded by posttranslational modification.
2013
Marcoleta, A., Marín, M., Mercado, G., Valpuesta, J.M., Monasterio, O, and Lagos, R.
J. Bacteriol. 195, 3995-4004.
Whole genome sequence of the microcin E492-producing strain Klebsiella pneumoniae RYC492.
2013
Marcoleta, A., Gutiérrez-Cortez, S., Maturana, D., Monasterio, O., and Lagos, R.
Genome Announc. 1(3):e00178-13. doi:10.1128/genomeA.00178-13.
Glutamate 83 and arginine 85 of helix H3 bend are key residues for FtsZ polymerization, GTPase activity and cellular viability of Escherichia coli: lateral mutations affect FtsZ polymerization and E. coli viability.
2013
Shin, J-Y., Vollmer, W., Lagos, R., and Monasterio, O.
BMC Microbiology, 13:26 doi:10.1186/1471-2180-13-26
Bacteriocins.
2013
Lagos, R., and
Brenner’s Online Encyclopedia of Genetics, Second edition, Vol.1 (Maloy, S. and Hughes, K., Editors) Academic Press. San Diego, USA, pp. 277-279.