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Lagos R. publications (last 10 years).

Identification of aggregation-prone and gatekeeper residues in bacterial amyloids using site-directed mutagenesis and flow cytometry.


Aguilera, P., Marín, J., Lagos, R., Marcoleta, A.

In Methods in Molecular Biology 2538.  Bacterial Amyloids: Methods and Protocols.   Eds: V. Arluison, F. Wien, A. Marcoleta.  Humana New York, NY. DOI:

Assessment of intracellular amyloid formation in fixed and live bacteria using fluorescence microscopy.


Marín, J., Aguilera, P., Lagos,R., Marcoleta, A.

In Methods in Molecular Biology 2538.  Bacterial Amyloids: Methods and Protocols.   Eds: V. Arluison, F. Wien, A. Marcoleta.  Humana New York, NY. DOI:

The highly diverse Antarctic Peninsula soil microbiota as a source of novel resistance genes.


Marcoleta, AE., Arros, P.,  Varas, MA,  Costa, J., Rojas-Salgado, J., Berríos-Pastén, C.,  Tapia-Fuentes, S., Silva, D., Fierro, J.,  Canales, N., Chávez, FP.,  Gaete, A.,   González, M., Allende, ML,  Lagos, R.

Science of the Total Environment. 810 (2022) 152003.

Exploiting zebrafish xenografts for testing the in vivo antitumorigenic activity of microcin E492 against human colorectal cancer cells.


Varas, M.A., Muñoz-Montecinos, C., Kallenz, V.,  Simon, V., Allende, M.A., Marcoleta, A.E. and Lagos, R.

Science of the Total Environment. 810 (2022) 152003.

Properties of genes encoding transfer RNAs as integration sites for genomic islands and prophages in Klebsiella pneumoniae.


Berríos-Pastén, C., Acevedo, R., Arros, P., Varas, MA.,  Wyres, KL., Lam, MMC., Holt, KE, Lagos, R., AE. Marcoleta

bioRxiv preprint doi:

Inhibition of Escherichia coli and Bacillus subtilis FtsZ Polymerization and Bacillus subtilis Growth by Dihydroxynaphtyl Aryl Ketones.


Araya G, Benites J, Reyes JS, Marcoleta AE, Valderrama JA, Lagos R and Monasterio O

Frontiers in Microbiology.10:1225. doi: 10.3389/fmicb.2019.01225

Bacterial Amyloids.


Marcoleta, A., Wein, F., Arluison, V., Lagos, R., Giraldo, R.

In eLS, John Wiley  & Sons. Ltd: Chichester. doi: 10.1002/9780470015902.a0028401

The Ferric uptake regulator (Fur) and iron availability control the production and maturation of the antibacterial peptide microcin E492.


Marcoleta, AE., Gutiérrez-Cortez, S., Hurtado, F.,  Argandoña,  Y., Corsini, G., Monasterio, O.,  and Lagos, R.

PLoS ONE 13(8): e0200835.

Evaluating different virulence traits of Klebsiella pneumoniae using the social amoeba Dictyostelium discoideum and zebrafish larvae as host models.


Marcoleta, A.E,  Varas, M.A, Ortiz-Severín, J. Vásquez, L., Berríos-Pastén,  C.,  Sabag, A., Chávez,  F.P., Allende, M.L., Santiviago, C.,  Monasterio, O.,  and R. Lagos.

Frontiers in Cellular and Infection Microbiology 8:30. doi: 10.3389/fcimb.2018.00030

Thermal Adaptation of Mesophilic and Thermophilic FtsZ Assembly by Modulation of the Critical Concentration.


Concha-Marambio, L., Maldonado, O.,  Lagos, R.,  Monasterio, O.,  Montecinos-Franjola, F.

PloS ONE.(10):  PLoS e0185707.

Bacterial Microtubules Exhibit Polarized Growth, Mixed-Polarity Bundling, and Destabilization by GTP Hydrolysis.


Díaz-Celis, C., Risca, V.I.,  Hurtado, F., Polka, J., Hansen, S.D., Maturana, D., Lagos, R., Mullins, D., Monasterio, O.

Journal of Bacteriology 199 (19) e0221-17.

Mutations on FtsZ lateral helix H3 that disrupt cell viability hamper reorganization of polymers on lipid surfaces.


Marquez, I.F.,  Mateos-Gil, P.,  Shin, J-Y., Lagos, R.,  Monasterio, O.,  Velez, M.

Biochim Biophys Acta  – Biomembranes. 1859, 1815-1827.

Inhibition of cancer cell growth and migration by dihydroxynaphthyl aryl ketones.  


Benites, J.,  Valderrama, J.A., Ríos, D.,  Lagos, R., Monasterio, O., and  Buc Calderon, P.

Molecular & Cellular Toxicology  (MCT) 12:  237-242. doi:10.1007/s13273-016-0028-8

Klebsiella pneumoniae asparagine tDNAs are integration hotspots for different genomic islands encoding microcin E492 production determinants and other putative virulence factors present in hypervirulent strains.


Marcoleta, A., Berríos-Pastén, C., Nuñez, G., Monasterio, O., Lagos, R.

Frontiers in Microbiology. 7:849  doi: 10.3389/fmicb.2016.00849

Identification of key amino acid residues modulating intracellular and in vitro microcin E492 amyloid formation.


Aguilera, P., Marcoleta, A., Lobos-Ruiz, P.,  Arranz, R., Valpuesta, JM., Monasterio, O., Lagos, R.

Frontiers in Microbiology. 7:35.  doi: 10.3389/fmicb.2016.00035.

Binding of dihydroxynaphtyl aryl ketones  to tubulin colchicine sites inhibits microtubule  assembly.


Gutiérrez , E., Benites, J., Valderrama, J., Buc-Calderon, P., Verrax, J., Nova E., Villanelo, F., Maturana, D., Escobar, C., Lagos, R., Monasterio, O.

Biochem. Biophys Res. Commun. 466: 418-425. doi: 10.1016/j.bbrc.2015.09.041.

Single tryptophan mutants of FtsZ: nucleotide binding/exchange and conformational transitions.


Montecinos-Franjola, F., James, N.G., Concha-Marambio, L., Brunet, J.E., Lagos, R., Monasterio, O., Jameson, D.M.

.  Biochim Biophys Acta 1844, 1193-200.

Microcin E492 amyloid formation is retarded by posttranslational modification.


Marcoleta, A., Marín, M., Mercado, G., Valpuesta, J.M., Monasterio, O, and Lagos, R.

J. Bacteriol. 195, 3995-4004.

Whole genome sequence of the microcin E492-producing strain Klebsiella pneumoniae RYC492.


Marcoleta, A., Gutiérrez-Cortez, S., Maturana, D., Monasterio, O., and Lagos, R.

Genome Announc. 1(3):e00178-13. doi:10.1128/genomeA.00178-13.

Glutamate 83 and arginine 85 of helix H3 bend are key residues for FtsZ polymerization, GTPase activity and cellular viability of Escherichia coli: lateral mutations affect FtsZ polymerization and E. coli viability.


Shin, J-Y., Vollmer, W., Lagos, R., and Monasterio, O.

BMC Microbiology, 13:26 doi:10.1186/1471-2180-13-26



Lagos, R., and 

Brenner’s Online Encyclopedia of Genetics, Second edition, Vol.1 (Maloy, S. and Hughes, K., Editors) Academic Press. San Diego, USA, pp. 277-279.

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